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KMID : 1144320190510040365
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감염과 화학요법
2019 Volume.51 No. 4 p.365 ~ p.375
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Characteristics of Faecal Microbiota in Korean Patients with Clostridioides difficile-associated Diarrhea
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Jeon Yong-Duk
Ann Hea-Won
Lee Woon-Ji
Kim Jun-Hyoung
Seong Hye
Kim Jung-Ho
Ahn Jin-Young
Jeong Su-Jin
Ku Nam-Su
Yeom Joon-Sup
Yong Dong-Eun
Lee Kyung-Won
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Abstract
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Background: The intestinal microbiota plays an important role in the pathogenesis of Clostridioides difficile-associated diarrhea, and regional and racial characteristics influence the microbiome composition and diversity. We investigated the intestinal microbiome characteristics of patients with C. difficile colitis (CD+) compared to those of patients with colitis not due to C. difficile (CD?), patients with vancomycin-resistant enterococci (VRE) colonization, and healthy controls, in Korea.
Materials and Methods: We collected stool samples from 24, 18, 11 and 13 subjects within CD+, CD?, VRE and healthy control groups, respectively. The microbial communities were evaluated by 454-pyrosequencing of bacterial 16s rRNA.
Results: The species richness and microbial diversity were significantly lower in the CD+ group compared to those in healthy controls, but not compared to those in CD? and VRE groups. Phylum-level analysis showed that the proportion of Actinobacteria in the CD+ group was significantly lower than in the healthy control, but was unchanged compared to that in CD? and VRE groups. At the genus level, compared to the healthy group, the CD+ group showed significantly lower proportions of Blautia, Bifidobacterium, Faecalibacterium et al. Compared to the VRE group, the CD+ group showed a significantly higher proportion of Anaerostipes.
Conclusions: We could identify the intestinal microbiome characteristics of Koreans with C. difficile colitis. It might help to develop microbiome based diagnostic and treatment modalities.
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KEYWORD
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Faecal microbiota, Intestinal microbiota, Clostridioides difficile infection, Next generation sequencing
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